gluconacetobacter diazotrophicus isolation
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0000004418 00000 n 0000113789 00000 n 0000005203 00000 n Bacterial cultures have been preserved in 20% glycerol at −80 °C prior to analysis. CAB Direct provides You can now claim your publications on CAB Direct with your ORCID iD! 0000038428 00000 n 0000058762 00000 n [7] Besides to be a nitrogen-fixing bacterium, G. diazotrophicus synthesizes Indole-3-acid acetic, that could contribute to promote the growth of the associated plant. [2] The bacterium has been studied to move from different crops and also helpful in growing corn. Gluconacetobacter- like diazotrophs were encountered as dense populations inside the root and stem tissues of sugarcane cultivated in ancient agricultural fields of the Nile Delta. [2][3] Again, the bacterium’s name was changed to Gluconacetobacter diazotrophicus when its taxonomic position was resolved using 16s ribosomal RNA analysis. 0000005981 00000 n 0000004655 00000 n 0000005512 00000 n Overall, Gluconacetobacter diazotrophicus, through the research restated, plays a significant role in the environment for plants specifically sugar cane, helps to grow crops, and can be found in areas that are acidic and contain oxygen. [2][3], Originally found in Alagoas, Brazil, Gluconacetobacter diazotrophicus is a bacterium that has several interesting features and aspects which are important to note. 0000003949 00000 n 0000006297 00000 n [10] The leaves contained a smaller population (<10 3 g -1). Gluconacetobacter diazotrophicus is a bacterium with a rod-like shape, has circular ends, and can be classified as a Gram-negative bacterium. 0000004733 00000 n 0000004811 00000 n 0000005744 00000 n 0000004576 00000 n 0000126977 00000 n 0000004071 00000 n For DNA isolation, a single bacterial colony of isolate DS1 was inoculated into 5ml of a rich medium (gl 1; glucose, 2.0; glutamic acid, 1.5; peptone, 1.5; K 2HPO 4, 0.5; MgSO 4 7H 2O, 0.5; yeast extract, 2.0; pH, 6). [1] Because they create phytohormones, Gluconacetobacter diazotrophicus has proven to stimulate other plant’s growth. 0000128321 00000 n 0000005434 00000 n 0000052542 00000 n 0000053931 00000 n [2] This genome has one circular chromosome and two plasmids. 0000065511 00000 n 0000042369 00000 n Strains of this species have been isolated from all over the world. [8] This microbe fights off Xanthomonas albilineans which is a pathogen found in sugar cane. 0000003296 00000 n 0000005823 00000 n 0000127870 00000 n 0000127391 00000 n [2] Gluconacetobacter diazotrophicus is a notable microbe because studies have shown that the bacteria can help tomatoes and other crops grow. [1][2] The bacterium is known for stimulating plant growth and being tolerant to acetic acid. 0000006060 00000 n Counts of >10 5 cells g -1 were recorded in root and stem samples. 0000005667 00000 n The bacterium is known for stimulating plant growth and being tolerant to acetic acid. [2] The two plasmids found in the genome are pGD01 and pGD02 which contain 38,818 and 16,610 base pairs respectively. 0000003706 00000 n Gluconacetobacter diazotrophicus cells are shaped like rods, have ends that are circular or round, and have anywhere from one to three flagella that are lateral. 0000042681 00000 n 0000042037 00000 n [9] In regard to the ecology of this microorganisms, the numbers of G. diazotrophicus that colonize sugarcane decrease when the plant is grown under high nitrogen fertilization doses. 0000128095 00000 n xref trailer Gluconacetobacter diazotrophicusis an endophytic bacterium first isolated from the sugarcane growing regions of Brazil (Cavalcante and Dobereiner, 1988). 0000040252 00000 n 0000003248 00000 n [1][2] In addition to sugarcane, G. diazotrophicus has been found in different plants like coffee tree and pineapple. [2] The Pal5 genome was found to have 583 proteins that can be used to depict the possible “metabolic pathways” in Gluconacetobacter diazotrophicus. 0000036832 00000 n [2] Other nitrogen-fixing species in this same genus include Gluconacetobacter azotocaptans and Gluconacetobacter johannae [4]. 0000000016 00000 n CAB Direct is the most thorough and extensive source of reference in the applied life sciences, Gluconacetobacter diazotrophicus is a bacterium with a rod-like shape, has circular ends, and can be classified as a Gram-negative bacterium. 0000127765 00000 n 114 0 obj<>stream 0000044911 00000 n 0000057404 00000 n 0000005046 00000 n 0000039542 00000 n 0000127496 00000 n With one to three lateral flagella, and known to be found on sugarcane, Gluconacetobacter diazotrophicus was discovered in Brazil by Vladimir A. Cavalcante and Johanna Dobereiner. <]>> [1][2] Based on these descriptions of the cell, Gluconacetobacter diazotrophicus can be classified with the bacillus genus. Find out more about this exciting new development, Using our new visualization tools you can, Using our new highlighting and annotation tool you can, remove selected records that are not saved in My CABI, sign you out of your 0000041289 00000 n After overnight growth at 301C, QIAGEN blood and cell culture DNA Midi kit … 0000034063 00000 n [2] In addition to being a part of the Acetobacter family, Gluconacetobacter diazotrophicus belongs to the Proteobacteria phylum, the Alphaprotebacteria class, and the Gluconacetobacter genus while being a part of the Rhodosprillales order.
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